vital stain for protoplasts

Tony Travis ajt at
Wed Jan 31 12:35:50 EST 1996

Alyson Tobin wrote:
> We are working with isolated barley leaf protoplasts and need to stain them
> to test for intactness of the plasmamembrane. I'm sure there is a suitable
> dye but can't remember the name of it . Can anyone help with a name,
> supplier and/or reference for the method please?
> Alyson Tobin (St Andrews University)

I think the stain you want is neutral red (available from Sigma).

Dr. A.J.Travis,                     |  mailto:ajt at
Rowett Research Institute,          |
Greenburn Road, Bucksburn,          |   phone:+44 (0)1224 712751
Aberdeen AB2 9SB, Scotland, UK.     |     fax:+44 (0)1224 716687

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