Arabidopsis fluorescence

Charles S Buer buer at WPI.EDU
Fri Jun 7 07:49:42 EST 1996


Dear netters--

I have been conducting some experiments with Arabidopsis and viewing the
callus tissue under a fluorescing microscope.  I am wondering if any one
can inform me what compound fluoresces yellow (cellulose ?) and which
fluoresces light green at 490 nm. I also have deep red but am sure that is
chlorophyll. The yellow occurs in older tissue and the light green in new
cells.  The callus is grown in light and is a light yellowish green in
color under ambient light.  The fluorescence occurs without adding any
chemicals such as fluorescein diacetate.

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Charles S. Buer                         |E-mail:  buer at wpi.wpi.edu
Biology/Biotechnology Dept.             |Phone:   (508) 831-5052
Worcester Polytechnic Institute         |FAX:     (508) 831-5936
100 Institute Rd.                       |http://www.wpi.edu/~buer
Worcester, MA 01609                     |
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