Seeking Molecular/Biochemistry Position
Ioannis M. Ioannides
iioannid at prairienet.org
Fri Jun 14 17:36:55 EST 1996
I am presently seeking a position in industry, research institute, or
academia, working in the areas of plant/bacterial biochemistry/molecular
biology. My experiences are from working with plant and microbial pigment
biochemistry and molecular biology of microbial enzymes. However, my broad
background allows me to work in multi-disciplinary applications. I have no
preferences toward geographical region, domestic or international.
CV Below
For inquiries please email me at the address below.
Kind regards,
Ioannis M. Ioannides, Ph.D.
iioannid at prairienet.org
Curriculum Vitae
Ioannis M. Ioannides
Division of Anaerobic Microbiology
Department of Animal Sciences
University of Illinois at Urbana-Champaign
1207 West Gregory Drive
Urbana, IL 61801 / U.S.A.
Tel: (217) 333-8809
FAX: (217) 333-8804
e-Mail: iioannid at prairienet.org
I. EDUCATION
Post Doctoral Molecular Biology / Anaerobic Microbiology
University of Illinois at Urbana-Champaign 11/93-Present
Doctorate Porphyrin Biochemistry
University of Illinois at Urbana-Champaign 1993
Master of Science Pigment Biochemistry
University of Illinois at Urbana-Champaign 1989
Bachelor of Science Plant Production
Aristotelian University of Thessaloniki 1986
II. PROFESSIONAL EXPERIENCE
Postdoctoral Research Associate 11/93-Present
University of Illinois at Urbana-Champaign
Graduate Research Assistant 1/87-11/93
University of Illinois at Urbana-Champaign
Research Assistant 1/83-2/86
Aristotelian University of Thessaloniki
Practical Training-Field Experience 4/83-9/83
Aristotelian University of Thessaloniki
III. RESEARCH EXPERIENCE
Molecular Biology
Genomic and Plasmid DNA Isolation, Nucleic Acid Hybridization, DNA Cloning,
Random Priming, End-labeling, Southern Blotting - RFLP Mapping, Northern
Blotting - RNA Isolation, Library Construction and Screening, Quantitative
RT-PCR, Inverse PCR, Genome-walking PCR, In vitro
Transcription-Translation, cDNA Preparation, DNA Sequence Analysis, Dideoxy
DNA Sequencing, Oligonucleotide Synthesis and Gene Transformation (CaCl2 -
Electroporation)
Biochemistry / Physiology
Enzymatic and Biochemical Assays, Use of Cell-Free Systems to Study
Biosynthetic Pathways, Kinetic Analysis of Biosynthetic Branching,
Purification of Tetrapyrroles (TLC, HPLC), Separation, Size Analysis and
Activity Staining of Proteins by Native and SDS-PAGE, Spectrophotometry,
Spectrofluorometry, 1H NMR Spectrometry, Mass Spectroscopy, Fourier
Transform Infrared (FTIR) Spectroscopy, X-ray Photoelectron Spectroscopy
and Elemental (C, H, N) Combustion Analysis
Microbiology
Anaerobic Culture Technology, Medium Preparation and Sterilization, Growth
Physiology, Cell Disruption Techniques, Enzymatic Assays, Fermentation
Systems, Batch Cultivation and Selective Enrichment
Computer Expertise
Gene Sequence Analysis, 3-D Molecular Modeling, Video Image Technology,
Knowledge Based Models and Expert Systems, Graphing and Statistical
Analysis, Slide and Photo Production and Word Processing
IV. ORGANIZATIONS and PROFESSIONAL SOCIETIES
President International Agriculture Student Association 4/92-8/93
Nominated for Outstanding Student
Organization Award for 1993
Crop Science Society of America
Gamma Sigma Delta-Honor Society of Agriculture
American Society of Microbiology
V. PUBLICATIONS
Shedbalkar, V. P., I. M. Ioannides, and C. A. Rebeiz. (1991). Chloroplast
biogenesis. Detection of monovinyl protochlorophyll(ide) b in plants.
Journal of Biological Chemistry. 226:17151-17157
Ioannides, I. M., K. R. Robertson, and C. A. Rebeiz. (1992). Chloroplast
biogenesis 65. Study of the greening group affiliation of green plants.
Plant Physiology (Life Sci. Adv.) 11:79-88
Fasoula, D. A. and I. M. Ioannides. (1992). Breeding significance and
measurement of the departure from normality of single-plant-yield frequency
distributions. 4th Congress of the Hellenic Scientific Society for the
Genetic Improvement of Plants
Rebeiz, C. A., R. Parham, D. A. Fasoula, and I. M. Ioannides. (1994).
Chlorophyll a biosynthetic heterogeneity. The biosynthesis of the
tetrapyrrole pigments. Ciba Foundation Symposium; pp. 177-193
Ioannides, I. M., D. A. Fasoula, K. R. Robertson, and C. A. Rebeiz. (1994).
An evolutionary study of chlorophyll biosynthetic heterogeneity of green
plants. Biochemical Systematics and Ecology. 22:211-220
Ioannides, I. M. and C. A. Rebeiz. (1996). Quantitative determination of
monovinyl protochlorophyllide b (ester) by spectrofluorometry. Analytical
Biochemistry [Submitted]
Ioannides, I. M. and C. A. Rebeiz. (1996). Development of a cell free
system capable of the net biosynthesis and accumulation of 2-MV chlorophyll
b in the light. Biochemistry [Submitted]
Rebeiz, C. A., B. R. Wade, A. J. Baublis, I. M. Ioannides, D. A. Fasoula,
K. E. Diehl, and C. Smyth. (1996). Semiemperical quantum mechanical
calculations of various descriptors of the monovinyl and divinyl
tetrapyrrole intermediates of the chlorophyll biosynthetic pathway [In
preparation]
Ioannides, I. M., P. E. Vercoe, B. A. White, and R. I. Mackie. (1996).
Cloning and sequencing of NADP-dependent glutamate dehydrogenase (GDH)
gene, from the cellulolytic ruminal bacterium Ruminococcus flavefaciens
FD-1 [In preparation]
Ioannides, I. M., M. D. Berber-Jimenez, B. A. White, and R. I. Mackie.
(1996). Characterization of the yellow fluorescent compound produced by
Ruminococcus flavefaciens during growth on cellulose [In preparation]
VI. ABSTRACTS
Ioannides, I. M., K. R. Robertson, and C. A. Rebeiz. (1991). Evolutionary
study of the greening group affiliation of green plants. American Journal
of Botany. 78 (6):193
Rebeiz, C. A., R. Parham, D. A. Fasoula, and I. M. Ioannides. (1993).
Chlorophyll a biosynthetic heterogeneity. The biosynthesis of the
tetrapyrrole pigments. Ciba Foundation Symposium No. 180
Fasoula, D. A., I. M. Ioannides, and V. A. Fasoula. (1993). A criterion for
selection among entries that considers jointly productivity, stabilidy and
heritability. Agronomy Abstracts. p. 88
Ioannides, I. M., M. D. Berber-Jimenez, B. A. White, and R. I. Mackie.
(1995). Purification and putative characterization of the yellow
fluorescent compound produced by Ruminococcus flavefaciens during growth on
cellulose. American Society for Microbiology. 95th Annual Meeting,
Washington DC
Ioannides, I. M., P. E. Vercoe, B. A. White, and R. I. Mackie. (1996).
Cloning and sequencing of NADP-dependent glutamate dehydrogenase (GDH)
gene, from the cellulolytic ruminal bacterium Ruminococcus flavefaciens
FD-1. American Society for Microbiology. 96th Annual Meeting, New Orleans
VII. SYMPOSIA ATTENDED
1994 Biotechnology Training Programs. Introduction to Polymerase Chain
Reaction (PCR) and Quantitative RT - PCR. Loyola University Medical
Center, Chicago, Illinois
1994 Applications of Molecular Techniques in Environmental
Engineering. University of Illinois, Urbana-Champaign
1994 Animal Sciences Graduate Student Retreat.
University of Illinois, Urbana-Champaign
1995 Animal Sciences Graduate Student Retreat.
University of Illinois, Urbana-Champaign
1995 Nitrogen Metabolism and Related Topics.
University of Illinois, Urbana-Champaign
1995 Bryant 70th Symposium. Rumen Microbiology, the Environmental
Application of Microorganisms and Biodiversity.
University of Illinois, Urbana-Champaign
VIII. SEMINARS and PRESENTATIONS
1989 Chlorophyll biosynthetic heterogeneity of green plants. Department of
Horticulture, University of Illinois, Urbana-Champaign
1991 Evolutionary study of the greening group affiliation of green plants.
American Institute of Biological Sciences meeting, San Antonio, Texas
1992 Intermediate metabolism of the chlorophyll b biosynthetic pathway.
Department of Horticulture, University of Illinois, Urbana-Champaign
1993 Metabolism of the 2-MV protochlorophyllide b. An intermediate of the
chlorophyll b biosynthetic pathway. Department of Horticulture, University
of Illinois, Urbana-Champaign
1994 Invited speaker: Chlorophyll degradation in the rumen. Department of
Animal Sciences, University of Illinois, Urbana-Champaign
1994 Invited speaker: Bacillus DNA in bees: A window to ancient symbiotic
relationships? Department of Animal Sciences, University of Illinois,
Urbana- Champaign
1994 Invited lecturer: Presented several lectures covering the topics of
Carbohydrates, TCA cycle and Glycolysis to an upper level under-graduate
biochemistry course. Department of Animal Sciences, University of
Illinois, Urbana-Champaign
1995 Invited speaker: Why does Escherichia coli have two primary pathways
for synthesis of glutamate? Department of Animal Sciences, University of
Illinois, Urbana-Champaign
1995 Invited lecturer: Presented several lectures covering the topics of
Overview of Intermediary metabolism, Carbohydrates, TCA cycle and
Glycolysis to an upper level under-graduate biochemistry course.
Department of Animal Sciences, University of Illinois, Urbana-Champaign
IX. REFERENCES
Roderick I. Mackie, Professor
Post doctoral advisor
Department of Animal Sciences
Division of Microbiology
458 Animal Sciences Laboratory
1207 West Gregory Drive
Urbana, IL 61801
Tel: (217) 244-2526
Bryan A. White, Associate Professor
Post doctoral co-advisor
Department of Animal Sciences
Division of Microbiology
460 Animal Sciences Laboratory
1207 West Gregory Drive
Urbana, IL 6180
Tel: (217) 333-2091
Constantin A. Rebeiz, Professor
Ph.D. advisor
Department of Natural Resources and Environmental Sciences
240b Plant and Animal Biotechnology Laboratory
1201 West Gregory Drive
Urbana, IL 61801
Tel: (217) 333-1968
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