Help! chloroplast isolation

Zhu Congju medp4076 at
Wed Jun 26 05:47:41 EST 1996

I tried several methods for chloroplast isotion. However, there always had
some problems in cell homogenisation step, in which, both plasmamembrance
and chloroplast membrane of a wall-less algal cell (Isochrysis) were
broken. Are there any protocols available for gentle repture of the cell, 
except French and Yeda press?

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