floating of protoplasts

Frank Maiwald maiwald at mpiz-koeln.mpg.de
Mon Nov 4 06:06:40 EST 1996


All barley and rice protoplasts that I isolate still contain
considerable amounts of cell wall material which I must get rid of
completely. I would like to include a floating step into my protocol.
Could anybody suggest a good concentration for the (sucrose) cushion for
the two cell types?

Thanks in advance					Frank

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