Postdoc Fellowship, Arabidopsis Photobiology, Bay Area/CA

Winslow Briggs BRIGGS at ANDREW.STANFORD.EDU
Tue Nov 12 15:50:39 EST 1996


Postdoctoral Fellowship Position in Arabidopsis Photobiology, Bay Area/California

Funds are available to fund a postdoctoral fellow working on
molecular and biochemical aspects of signal transduction in
phototropism in Arabidopsis and other higher plants, beginning early
in 1997. Experience in a range of techniques in molecular biology
and biochemistry desirable. Please send your resume and arrange to
have three letters of reference forwarded to Dr. Winslow R. Briggs,
Department of Plant Biology, Carnegie Institution of Washington, 290
Panama St., Stanford, CA 94305. 

We have been studying a plant plasma membrane protein that becomes
heavily phosphorylated on irradiation with blue light either in vivo
or in vitro. Physiological and genetic evidence implicate a role for
the protein early in the signal transduction pathway for
phototropism, and photobiological and biochemical studies suggest
that it may be the photoreceptor for phototropism. Mutants deficient
in or lack the protein (nph1 for non-phototropic hypocotyl) have
been used to clone the gene via AFLP and sequencing is currently in
progress. 

We plan to attempt to rescue nph1 mutant alleles by transformation
with the wild-type gene in the near future, and we plan to use the
cloned gene a) to study the effects of overexpression on
phototropism physiology and the physiology of a number of other blue
light-sensitive phenomena; b) as a probe to clone and characterize
the NPH1 gene from other species; c} as a probe to search for
related genes in Arabidopsis; d) to investigate NPH1 expression at
the mRNA and protein levels in different tissues during development
and during various light/dark regimes; and e) to look for reaction
partners with NPH1 using cross-linking reagents, two-hybrid
complementation, etc. to investigate further elements in the signal
transduction pathway.

We plan to construct an expression systems a) to do further
biochemical characterization of the protein; b) to study potential
chromophore association; c) to generate polyclonal and monoclonal
antibodies; d) to investigate possible mechanisms of activation of
phosphorylation if the expressed protein shows photoactivity; and e)
to use it as a starting point for site-directed mutagenesis studies.

Antibodies will allows to investigate a) protein distribution in
various tissues during development; b) the fate of the protein
following phosphorylation; c) the behavior of the protein in
light-grown plants; and d) the possible occurrence of the protein in
lower plants and fungi. 
Winslow R. Briggs
Phone (415) 325-1521 Ex 207
Fax (415) 325-6857
email briggs at andrew.stanford.edu



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