sterilizing media

James Aldridge jaldr at onramp.net
Tue Nov 19 21:36:23 EST 1996


I was going to try some plant tissue work, and I have a question about
media sterilization. I'm using baby food jars with Magenta caps w/
millipore vents. 

As the sterilizer (a stove top unit) cools and is finally vented, unsterile
air is drawn up into the chamber and, I suppose, into the baby food jars
(the caps don't fully seal the threads). Should I be worrying about this?
Is there any way to minimize the problem?

Thank you.

James Aldridge - Fort Worth, Texas, USA
jaldr at onramp.net or aldrjame at tenet.edu
http://rampages.onramp.net/~jaldr



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