Couple seeks two postdoc positions in plant science/molecular biology

[Stanislav Kopriva]

To whom it may concern:

In the moment, I and my girlfriend are looking for postdoctoral positions
in plant science/molecular biology, starting by December 96 - February
97. Now we are both working in the Institute of Plant Genetics and Crop
Plant Research, Gatersleben, Germany and we are prepared to relocate, but
only together.
We both have experience in molecular biology, PCR methods, gene cloning
and expression analysis, expression of proteins in E.coli, transformation
of plants etc.. Furthemore we can guarantee an excellent cooperation
between us and also with other scientists.

Our resumes follow.

Thank you in advance.

RNDr Stanislav Kopriva
Dept. of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany



Curriculum Vitae 1

RNDr Stanislav Kopriva, born on March 13, 1967 in Ceske Budejovice, Czech =

Republic
present adress: Department of Molecular Cell Biology
                          Institute of Plant Genetics and Crop Plant =

Research
                          06466 Gatersleben
                          Germany
Phone:   +49-39482-5363
Telefax:  +49-39482-5366
E-mail:     kopriva at ipk-gatersleben.de

Education:
Charles University, Department of Biochemistry, Prague 1985-1990
- Graduation with honors, M.Sc., 1990
- Dean=B4s Award for Outstanding Results, 1990
- Diplom Thesis: Interaction of cytochrom P-450IIB1 with =

fluoresceinisothiocyanate
- Research Assistentship, Dept. of Biochemistry, 1987-1989
Grammar School Prague, 1981-1985
- Student research work: Influence of forest age on the amount of =

nitrogen in water, 1985

Employment:
Research fellow, Department of Molecular Cell Biology, Protein =

Biochemistry group,  Institute of Plant Genetics and Crop Plant Research, =

Gatersleben, Germany, 1996-now
- research on Calvin cycle enzymes pentose phosphate-3-epimerase and =

transketolase, sequence analysis,   expression analysis, expression in E. =

coli, in vitro mutagenesis, study of the catalytic mechanism, analysis   =

of anti-epimerase transgenic plants. =

Postgraduate Student, Department of Molecular Cell Biology, =

Photosynthesis group, Institute of Plant Genetics and Crop Plant =

Research, Gatersleben, Germany, 1992-1995
- Ph.D. Thesis: The glycine decarboxylase multienzyme complex of =

Flaveria: Expression study and phylogenetic   analysis, submitted to =

Martin Luther University Halle, May 1996 expected time of defense - =

November 96
- research on expression of GDC in plants with different types of =

photosynthesis (C3, C3-C4, and C4), sequence   analysis of cDNAs encoding =

GDC, and phylogenetic analysis of the Flaveria genus based on cDNA =

sequences of   H-subunit of GDC.
- published 12 articles and 8 poster presentations (list of publication =

included) =

Research Fellow, Department of Biochemistry, Charles University, Prague =

1990-1991
- research on cytochrom P-450: isolation from rats, rabbits, and carps, =

selective FITC labelling, peptid   mapping, enzymatic analysis
- supervising undergraduate practical training
Main research achievements:
- Isolation and sequence analysis of cDNA clones for three subunits of =

glycine decarboxylase and serine   hydroxymethyltransferase and =

comparison of their expression in Flaveria species with different types =

of   photosynthesis =

- Identification and characterization of alternative splicing of =

H-protein pre-mRNA in C4  species of Flaveria
- Characterization of tissue-specific expression of multiple genes coding =

for P-protein and H-protein of GDC in   Flaveria pringlei.
- The first evidence of phylogenetically intermediate position of C3-C4 =

intermediate photosynthesis
- Isolation and characterization of cDNA clones coding for =

pentosephosphate-3-epimerase and transketolase   multigene family. This =

is the first eukaryotic epimerase and the first transketolase from higher =

plants
- expression of active PPE in E. coli, in vitro mutagenesis, isolation of =

mutated proteins, analysis of the   enzyme catalytic activity

Research skills:
cDNA library construction and screening, manual sequencing, sequence =

analysis, DNA, RNA, and protein isolation, Southern, Northern, and =

Western blotting, PCR, RT-PCR, competitive PCR, molecular cloning, =

preparation of constructs for protein expression in E. coli and for plant =

transformation, analysis of transgenic plants, in vitro mutagenesis, =

phylogenetic analysis, HPLC, et cetera.

Research interests:
Photorespiration, evolution of C4 photosynthesis, phylogenetic analysis, =

control and mechanism of alternative splicing, regulation of cell-type =

specific gene expression, RNA editing, cytochrome P-450, cancerogenesis

Other skills:
good active knowledge of four languages: Czech, English, Russian, and =

German
 =


List of publications

=B7 Kopriva S. and Bauwe H.: T-protein of Glycine Decarboxylase from =

Solanum tuberosum. Plant Physiol. 104:   1079-1080 (1994)
=B7 Kopriva S. and Bauwe H.: P-protein of Glycine Decarboxylase from =

Flaveria pringlei. Plant Physiol. 104:   1077-1078 (1994)
=B7 Kopriva S. and Bauwe H.: Serine hydroxymethyltransferase from Solanum =

tuberosum. Plant Physiol. 107: 271-272   (1995)
=B7 Bauwe H. and Kopriva S.: The gdcsPA Gene from Flaveria pringlei =

(Asteraceae). Plant Physiol. 107: 655 (1995)
=B7 Kopriva S., Turner S.R., Rawsthorne S. and Bauwe H.: T-protein of the =

Glycine Decarboxylase Multienzyme   Complex: Evidence for partial =

similarity to formyltetrahydrofolate synthetase. Plant Mol. Biol. 27: =

1215-1220   (1995)
=B7 Kopriva S., Cossu R., Bauwe H.: Alternative splicing results in two =

different transcripts for H-protein of   the glycine cleavage system in =

the C4 species Flaveria trinervia. Plant J. 8(3): 435-441 (1995) =

=B7 Bauwe H., Chu C.-C., Kopriva S., Nan Q.: Complete structure and =

expression analysis of the gdcsPA and gdcsPB   genes encoding two =

P-isoproteins of the glycine cleavage system from Flaveria pringlei. Eur. =

J. Biochem. 234:   116-124 (1995)
=B7 Kopriva S. and Bauwe H.: H-protein of glycine decarboxylase is encoded =

by multigene families in Flaveria   pringlei and F. cronquistii =

(Asteraceae). Mol. Gen. Genet. 249: 111-116 (1995)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Chloroplast =

pentose-5-phosphate 3-epimerase from potato: Cloning,   cDNA sequence, =

and tissue-specific enzyme accumulation. FEBS Lett. 377: 349-352 (1995)
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative =

splicing of H-protein of glycine   decarboxylase in C4 Flaveria species. =

In: Mathis P. (ed) Photosynthesis: from Light to Biosphere, Vol III,   =

655-658 (1995)
=B7 Kopriva S., Chu C.-C., Bauwe H.: H-protein of the glycine cleavage =

system in Flaveria: Alternative splicing   of the pre-mRNA occurs =

exclusively in advanced C4 species of the     genus. Plant J. 10: 369-373 =

(1996)
=B7 Kopriva S., Chu C.-C., Bauwe H.: Molecular phylogeny of Flaveria as =

deduced from the analysis of H-protein   nucleotide sequences. Plant Cell =

Environ. 19: 1028-1036 (1996)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Molecular cloning, =

sequencing, and expression analysis of   chloroplast transketolase from =

potato. Evidence that oxidative pentose phosphate cycle does not exist in =

  non-photosynthesising plant organs. J. Biol. Chem. submitted


Lectures

=B7 Kopriva S.: =84Phylogenetic analysis of Flaveria based on H-protein cDN=
A =

sequences=93, IPK Gatersleben, 10.4.1995
=B7 Kopriva S.: =84The glycine decarboxylase multienzyme complex from =

Flaveria: Expression study and phylogenetic   analysis=93, Institute of =

Plant Science, ETH Z=FCrich, 12.8.1996


Posters =


=B7 Kopriva S., Chu C., Deng Z., Cossu R., Bauwe H.: Glycine Decarboxylase =

complex in Flaveria: A comparison of   gdcH genes in species with =

different degrees of C4 photosynthesis.  Gordon Research Conference - CO2 =

    fixation, October 1993, Irsee
=B7 Kopriva S., Bauwe H.: Glycine decarboxylase complex in plants: =

Molecular cloning and primary structure of the   T-protein from P. =

sativum, S. tuberosum, and F. pringlei.  Gordon Research Conference - CO2 =

fixation, October   1993, Irsee
=B7 Kopriva S., Cossu R., Bauwe H.: cDNA cloning and analysis of gcsH, =

gcsP, gcsT, and shmt genes from Flaveria   species with different types =

of photosynthesis. 7.Tagung =84Molekularbiologie des Pflanzen=93, March 199=
4, =

  Wernigerode
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase in Flaveria. =

Arbeitstagung des SFB, May 1994, Halle
=B7 Bauwe H., Kopriva S.: Glycine decarboxylase from plants: Cloning of the=
 =

gdcsPA gene from Flaveria pringlei, a   C3 species and some properties of =

the corresponding protein. 4th International Congress Plant  Molecular   =

Biology, June 1994, Amsterdam
=B7 Chu C., Cossu R., Kopriva S., Deng Z., Bauwe H.: A comparison of GDCSH =

genes from Flaveria. 4th International   Congress Plant Molecular =

Biology, June 1994, Amsterdam
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase multienzyme =

complex from Flaveria, Arbeitstagung des   SFB, May 1995, Halle
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative =

splicing of H-protein of glycine   decarboxylase in C4 Flaveria species. =

Xth International Photosynthesis Congress, August 1995, Montpellier
 =


List of referees:

Dr. Hermann Bauwe                                     =

Biochemistry and Physiology Department
IACR-Rothamsted
Harpenden, Herts. AL5 2JQ, UK
Phone:     01582-763133
Telefax:   01582-760981
E-mail:     hermann.bauwe at bbsrc.ac.uk

Dr. Karl-Heinz S=FCss
Dept. of Molecular Cell Biology
Inst. of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:  039482-5363
Telefax: 039482-5366

Dr. Steve Rawsthorne
Dept. of Brassica and Oilseed Research
Cambridge Laboratory
John Innes Centre
Norwich NR4 7UH, UK
Phone:   01603-452571
Telefax:  01603-259882
E-mail:   steve.rawsthorne at bbsrc.ac.uk




CURRICULUM VITAE 2

Name: Anna V. Zavgorodnyaya, Ph.D.
Place of Birth: Lutsk, Volyn Province, UKRAINE.
Date of Birth: October 28, 1967.
Nationality: Russian.
Marital status: divorced.

Address for correspondence:
Dr. Anna V. Zavgorodnyaya
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:   +49-39482-5318
Telefax:  +49-39482-5366
E-mail:     zavgorod at ipk-gatersleben.de



Employment:

1995- present:      Institute of Plant Genetics and Crop Plant Research, =

Gatersleben, Germany
                             research fellowship.
                             - regulation of chlorophyll biosynthesis
                             - effect of herbicide treatment
                             - expression in E. coli and in vitro =

mutagenesis of ALA synthase

1991 - 1995:         Institute of Cell Biology and Genetic Engineering,
                              National Academy of Science of Ukraine, =

Kiev, UKRAINE.
                               Scientific Researcher.
                             - Ph. D. Thesis: =84Nucleo-cytoplasmic =

inheritance in asymmetric somatic    =

                               hybrids in Lycopersicon genus".


Education:

1985-1990           Department of Genetics, Kiev State University, Kiev, =

UKRAINE.
                            - 1990 - M.Sc. Genetics.
                            -  Diplom Thesis: =84Effect of DNAseI treatment=
 =

on different evolutionary stadia =

                               of Drosophilla=93.
                            -  Scientific Researcher Assistant, Institute =

of Cell Biology and Genetic =

                               Engineering, National Academy of Science =

of Ukraine, Kiev, 1988-1990





Main research achievements:

- Complementation of GSA-AT antisense mutations by transformation with =

yeast ALA synthase =

- expression of active ALA-S in tobacco plants
- production of gabaculline tolerant tobacco plants
- investigation of influence of gabaculine and ALA on photosynthesis and =

expression of related proteins =

- first complex analysis of inheritance of organell and nuclear encode =

genes in fertile asymmetric somatic   hybrids between Lycopersicon =

esculentum and L. peruvianum.
- evidence for rearrangements in chloroplast genome of this hybrids

Research skills:

Southern, Northern, Western analysis; DNA, RNA, protein isolation; =

molecular cloning, restriction analysis; preparation of constructs for =

protein expression and for antisense and sense plant transformation; =

analysis of transgenic plants; PCR; in vitro mutagenesis; enzyme assays; =

isolation of organells from plants; work with radioisotops and in vitro =

cultures; somatic hybridization; isolation of chloroplast and =

mitochondrial DNA

Scientific interests:

Chlorophyll biosynthesis and its regulation; relation of protein =

structure and function; effect of herbicides on plants; genetic =

ingeneering; genetic transformation of plants; recombination of =

cytoplasmic genomes; nucleo-cytoplasmic interractions; RNA editing in =

plant organells; cancerogenesis.

Languages:

English, Russian, Ukrainian - read / speak.


List of publications

Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba =

YY (1991) Changes of the genetic constitution of the tomato plants by =

gamma-fusion of Lycopersicon esculentum Mill. and Lycopersicon peruvianum =

var. dentatum Dun. protoplasts. Biopolymers and Cell  7:82-91.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko  IV, =

 Rachkovskaya  RI,  Gleba  YY  (1993)  Cell engineering synthesis of =

tomato fertile asymmetric somatic hybrids by gamma-fusion of protoplasts. =

 Genetika,  29:164-176.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko IV, =

 Rachkovskaya RI,  Gleba YY  (1993)  Fertile asymmetric somatic hybrids =

between Lycopersicon esculentum  Mill. and Lycopersicon peruvianum var.  =

dentatum Dun. Mol. Gen. Genet, 236: 427-432.

Rudas  VA,  Zavgorodnyaya AV,  Cherep NN,  Gleba YY (1993) Genetic =

transformation by leaf-disks method of wild species Solanum and =

Lycopersicon. Biopolymers and  Cell  9:73-77.

Ratushnyak YI, Kochevenko SA, Cherep NN, Zavgorodnyaya AV, Latypov SA, =

Gleba YY (1995) Alloplasmic incompatibility of cybrid plants possest =

nuclear Lycopersicon esculentum and plasmagenes of Lycopersicon =

peruvianum var. dentatum Dun. Genetica 31(5): 660-667.

Zavgorodnyaya AV  (1994) Nucleo-cytoplasmic inheritance in asymmetric =

somatic hybrids in Lycopersicon genus. Ph.D. thesis, Kiev, UKRAINE.

Cherep NN, Zavgorodnyaya AV, Ratushnyak YI, Gleba YY (1995) =

Reconstruction of chloroplast DNA in asymmetric somatic hybrids =

Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum =

Dun.  Genetica  (in press).

Zavgorodnyaya AV, Grimm B (1996) Yeast 5-aminolevulinate synthase =

provides additional chlorophyll precursor in transgenic tobacco =

(submitted EMBO J.).

Zavgorodnyaya AV, N.N., Grimm B (1996) Influence of gabaculline on =

expression of proteins involved in chlorophyll biosynthesis and =

photosynthesis (manuscript in preparation).

ABSTRACTS:

Piven NM, Ratushnyak YI, Rudas VA, Latypov SA, Zavgorodnyaya AV,  Gleba  =

YY  (1991)  Selection  of  somatic  hybrids in  Lycopersicon and Solanum =

using chlorophyll-deficient mutants and gamma-irradiation.  Abstr  VIII  =

Internl  Protoplasts Symposium, Uppsala, p 28.

Cherep  NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba YY    (1993)  =

Chloroplast  DNA  rearrangement in Lycopersicon asymetric somatic =

hybrids.  Abstract  XVIIth  Intern.  Congress of Genetics, Birmingham, p =

191.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA,  Kochevenco =

AS, Gleba YY (1994) Alloplazmic incompatibility in cybrid plant of tomato =

obtained by cell engineerihg methods.  Abstract VIIIth Intern. Congress =

of Plant Tissue and Cell Culture, Firenze, p 101.

Cherep NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba  YY  (1994)  =

Organell and nuclear genomes analysis of tomato cybrid plants.  Abstracts =

4th Intern.  Congress  of  Plant  Molecular Biology, Amsterdam, p 153.


LECTURES:

ETH Z=FCrich, 12.8.1996: =84Yeast 5-aminolevulinate synthase provides =

additional chlorophyll precursor in transgenic tobacco=93



References:

Grimm Bernhard, Dr. hab.
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Tel.:  49-39482-5364
Fax:  49-39482-5139
E-mail: grimm at ipk-gatersleben.de

Gleba, Y.Y., Professor, Ph.D.
Director of Institute of Cell Biology and Genetic Engineering,
American Cyanamid Company,
Princeton, NJ 08540, =

U.S.A.
Tel: 1-609-799-0400.
Fax: 1-609-799-1842.
E-mail: glebay at pt.cyanamid.com

Alisher Touraev, Ph.D.
Institute of Mikrobiology and Genetics,
Biocentre of Bohrgasse, Vienna University =

Dr.Bohrgasse 9 =

1030 Vienna =

Austria.
Tel: 1-79515 7820.
Fax: 1-798 62 24.