Couple seeks two postdoc positions in plant science/molecular biology

Stanislav Kopriva kopriva at IPK-Gatersleben.de
Mon Sep 30 13:49:48 EST 1996


To whom it may concern:

In the moment, I and my girlfriend are looking for postdoctoral positions =

in plant science, starting by December 96 - February 97. Now we are both =

working in the Institute of Plant Genetics and Crop Plant Research, =

Gatersleben, Germany and we are prepared to relocate, but only together.
We both have experience in molecular biology, PCR methods, gene cloning =

and expression analysis, expression of proteins in E.coli, transformation =

of plants etc.. Furthemore we can guarantee an excellent cooperation =

between us and also with other scientists.

Our resumes follow.

Thank you in advance.

RNDr Stanislav Kopriva
Dept. of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research   =

06466 Gatersleben
Germany



Curriculum Vitae 1:
RNDr Stanislav Kopriva, born on March 13, 1967 in Ceske Budejovice, Czech =

Republic
present adress: Department of Molecular Cell Biology
                Institute of Plant Genetics and Crop Plant Research
                06466 Gatersleben
                Germany
Phone:   +49-39482-5363
Telefax:  +49-39482-5366
E-mail:   kopriva at ipk-gatersleben.de

Education:
Charles University, Department of Biochemistry, Prague 1985-1990
- Graduation with honors, M.Sc., 1990
- Dean=B4s Award for Outstanding Results, 1990
- Diplom Thesis: Interaction of cytochrom P-450IIB1 with =

fluoresceinisothiocyanate
- Research Assistentship, Dept. of Biochemistry, 1987-1989
Grammar School Prague, 1981-1985
- Student research work: Influence of forest age on the amount of =

nitrogen in water, 1985

Employment:

Research fellow, Department of Molecular Cell Biology, Protein =

Biochemistry group,  Institute of Plant Genetics and Crop Plant Research, =

Gatersleben, Germany, 1996-now
- research on Calvin cycle enzymes pentose phosphate-3-epimerase and =

transketolase, sequence analysis,     expression analysis, expression in =

E. coli, in vitro mutagenesis, study of the catalytic mechanism, analysis =

  of anti-epimerase transgenic plants. =


Postgraduate Student, Department of Molecular Cell Biology, =

Photosynthesis group, Institute of Plant Genetics and Crop Plant =

Research, Gatersleben, Germany, 1992-1995
- Ph.D. Thesis: The glycine decarboxylase multienzyme complex of =

Flaveria: Expression study and phylogenetic   analysis, submitted to =

Martin Luther University Halle, May 1996 expected time of defense - =

November 96
- research on expression of GDC in plants with different types of =

photosynthesis (C3, C3-C4, and C4), sequence   analysis of cDNAs encoding =

GDC, and phylogenetic analysis of the Flaveria genus based on cDNA =

sequences of   H-subunit of GDC.
- published 12 articles and 8 poster presentations (list of publication =

included) =


Research Fellow, Department of Biochemistry, Charles University, Prague =

1990-1991
- research on cytochrom P-450: isolation from rats, rabbits, and carps, =

selective FITC labelling, peptid   mapping, enzymatic analysis
- supervising undergraduate practical training


Main research achievements:
- Isolation and sequence analysis of cDNA clones for three subunits of =

glycine decarboxylase and serine   hydroxymethyltransferase and =

comparison of their expression in Flaveria species with different types =

of   photosynthesis =

- Identification and characterization of alternative splicing of =

H-protein pre-mRNA in C4  species of Flaveria
- Characterization of tissue-specific expression of multiple genes coding =

for P-protein and H-protein of GDC in   Flaveria pringlei.
- The first evidence of phylogenetically intermediate position of C3-C4 =

intermediate photosynthesis
- Isolation and characterization of cDNA clones coding for =

pentosephosphate-3-epimerase and transketolase   multigene family. This =

is the first eukaryotic epimerase and the first transketolase from higher =

plants
- expression of active PPE in E. coli, in vitro mutagenesis, isolation of =

mutated proteins, analysis of the   enzyme catalytic activity, proposal =

of catalytic mechanism.

Research skills:
cDNA library construction and screening, manual sequencing, sequence =

analysis, DNA, RNA, and protein isolation, Southern, Northern, and =

Western blotting, PCR, RT-PCR, competitive PCR, molecular cloning, =

preparation of constructs for protein expression in E. coli and for plant =

transformation, analysis of transgenic plants, in vitro mutagenesis, =

phylogenetic analysis, HPLC, et cetera.

Research interests:
Photorespiration, evolution of C4 photosynthesis, phylogenetic analysis, =

control and mechanism of alternative splicing, regulation of cell-type =

specific gene expression, RNA editing, cytochrome P-450, cancerogenesis, =

cell development

Other skills:
good active knowledge of four languages: Czech, English, Russian, and =

German
 =


List of publications

=B7 Kopriva S. and Bauwe H.: T-protein of Glycine Decarboxylase from =

Solanum tuberosum. Plant Physiol. 104:   1079-1080 (1994)
=B7 Kopriva S. and Bauwe H.: P-protein of Glycine Decarboxylase from =

Flaveria pringlei. Plant Physiol. 104:   1077-1078 (1994)
=B7 Kopriva S. and Bauwe H.: Serine hydroxymethyltransferase from Solanum =

tuberosum. Plant Physiol. 107: 271-272   (1995)
=B7 Bauwe H. and Kopriva S.: The gdcsPA Gene from Flaveria pringlei =

(Asteraceae). Plant Physiol. 107: 655 (1995)
=B7 Kopriva S., Turner S.R., Rawsthorne S. and Bauwe H.: T-protein of the =

Glycine Decarboxylase Multienzyme   Complex: Evidence for partial =

similarity to formyltetrahydrofolate synthetase. Plant Mol. Biol. 27: =

1215-1220   (1995)
=B7 Kopriva S., Cossu R., Bauwe H.: Alternative splicing results in two =

different transcripts for H-protein of   the glycine cleavage system in =

the C4 species Flaveria trinervia. Plant J. 8(3): 435-441 (1995) =

=B7 Bauwe H., Chu C.-C., Kopriva S., Nan Q.: Complete structure and =

expression analysis of the gdcsPA and gdcsPB   genes encoding two =

P-isoproteins of the glycine cleavage system from Flaveria pringlei. Eur. =

J. Biochem. 234:   116-124 (1995)
=B7 Kopriva S. and Bauwe H.: H-protein of glycine decarboxylase is encoded =

by multigene families in Flaveria   pringlei and F. cronquistii =

(Asteraceae). Mol. Gen. Genet. 249: 111-116 (1995)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Chloroplast =

pentose-5-phosphate 3-epimerase from potato: Cloning,   cDNA sequence, =

and tissue-specific enzyme accumulation. FEBS Lett. 377: 349-352 (1995)
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative =

splicing of H-protein of glycine   decarboxylase in C4 Flaveria species. =

In: Mathis P. (ed) Photosynthesis: from Light to Biosphere, Vol III,   =

655-658 (1995)
=B7 Kopriva S., Chu C.-C., Bauwe H.: H-protein of the glycine cleavage =

system in Flaveria: Alternative splicing   of the pre-mRNA occurs =

exclusively in advanced C4 species of the genus. Plant J. 10: 369-373 =

(1996)
=B7 Kopriva S., Chu C.-C., Bauwe H.: Molecular phylogeny of Flaveria as =

deduced from the analysis of H-protein   nucleotide sequences. Plant Cell =

Environ. in press (1996)
=B7 Teige M., Kopriva S., Bauwe H., S=FCss K.-H.: Molecular cloning, =

sequencing, and expression analysis of   chloroplast transketolase from =

potato. Evidence that oxidative pentose phosphate cycle does not exist in =

  non-photosynthesising plant organs. J. Biol. Chem. submitted
 =

Lectures

=B7 Kopriva S.: =84Phylogenetic analysis of Flaveria based on H-protein cDN=
A =

sequences=93, IPK Gatersleben, 10.4.1995
=B7 Kopriva S.: =84The glycine decarboxylase multienzyme complex from =

Flaveria: Expression study and phylogenetic   analysis=93, Institute of =

Plant Science, ETH Z=FCrich, 12.8.1996


Posters =


=B7 Kopriva S., Chu C., Deng Z., Cossu R., Bauwe H.: Glycine Decarboxylase =

complex in Flaveria: A comparison of   gdcH genes in species with =

different degrees of C4   photosynthesis.  Gordon Research Conference - =

CO2   =

  fixation, October 1993, Irsee
=B7 Kopriva S., Bauwe H.: Glycine decarboxylase complex in plants: =

Molecular cloning and primary structure of the   T-protein from P. =

sativum, S. tuberosum, and F. pringlei.  Gordon Research Conference - CO2 =

fixation, October   1993, Irsee
=B7 Kopriva S., Cossu R., Bauwe H.: cDNA cloning and analysis of gcsH, =

gcsP, gcsT, and shmt genes from Flaveria   species with different types =

of photosynthesis. 7.Tagung =84Molekularbiologie des Pflanzen=93, March 199=
4, =

  Wernigerode
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase in Flaveria. =

Arbeitstagung des SFB, May 1994, Halle
=B7 Bauwe H., Kopriva S.: Glycine decarboxylase from plants: Cloning of the=
 =

gdcsPA gene from Flaveria pringlei, a   C3 species and some properties of =

the corresponding protein. 4th International Congress Plant  Molecular   =

Biology, June 1994, Amsterdam
=B7 Chu C., Cossu R., Kopriva S., Deng Z., Bauwe H.: A comparison of GDCSH =

genes from Flaveria. 4th International   Congress Plant Molecular =

Biology, June 1994, Amsterdam
=B7 Kopriva S., Cossu R., Bauwe H.: Glycine decarboxylase multienzyme =

complex from Flaveria, Arbeitstagung des   SFB, May 1995, Halle
=B7 Kopriva S., Cossu R., Chu C.-C., Bauwe H.: Tissue specific alternative =

splicing of H-protein of glycine   decarboxylase in C4 Flaveria species. =

Xth International Photosynthesis Congress, August 1995, Montpellier
 =


List of referees:

Dr. Hermann Bauwe                                     =

Biochemistry and Physiology Department
IACR-Rothamsted
Harpenden, Herts. AL5 2JQ, UK
Phone:     01582-763133
Telefax:   01582-760981
E-mail:     hermann.bauwe at bbsrc.ac.uk

Dr. Karl-Heinz S=FCss
Dept. of Molecular Cell Biology
Inst. of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:  039482-5363
Telefax: 039482-5366

Dr. Steve Rawsthorne
Dept. of Brassica and Oilseed Research
Cambridge Laboratory
John Innes Centre
Norwich NR4 7UH, UK
Phone:   01603-452571
Telefax:  01603-259882
E-mail:   steve.rawsthorne at bbsrc.ac.uk




CURRICULUM VITAE 2

Name: Anna V. Zavgorodnyaya, Ph.D.
Place of Birth: Lutsk, Volyn Province, UKRAINE.
Date of Birth: October 28, 1967.
Nationality: Russian.
Marital status: divorced.

Address for correspondence:
Dr. Anna V. Zavgorodnyaya
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Phone:   +49-39482-5318
Telefax:  +49-39482-5366
E-mail:     zavgorod at ipk-gatersleben.de



Employment:

1995- present:      Institute of Plant Genetics and Crop Plant Research, =

Gatersleben, Germany
                             research fellowship.
                             - regulation of chlorophyll biosynthesis
                             - effect of herbicide treatment
                             - expression in E. coli and in vitro =

mutagenesis of ALA synthase

1991 - 1995:         Institute of Cell Biology and Genetic Engineering,
                              National Academy of Science of Ukraine, =

Kiev, UKRAINE.
                               Scientific Researcher.
                             - Ph. D. Thesis: =84Nucleo-cytoplasmic =

inheritance in asymmetric somatic    =

                               hybrids in Lycopersicon genus".


Education:

1985-1990           Department of Genetics, Kiev State University, Kiev, =

UKRAINE.
                            - 1990 - M.Sc. Genetics.
                            -  Diplom Thesis: =84Effect of DNAseI treatment=
 =

on different evolutionary stadia =

                               of Drosophilla=93.
                            -  Scientific Researcher Assistant, Institute =

of Cell Biology and Genetic =

                               Engineering, National Academy of Science =

of Ukraine, Kiev, 1988-1990





Main research achievements:

- Complementation of GSA-AT antisense mutations by transformation with =

yeast ALA synthase =

- expression of active ALA-S in tobacco plants
- production of gabaculline tolerant tobacco plants
- investigation of influence of gabaculine and ALA on photosynthesis and =

expression of related proteins =

- first complex analysis of inheritance of organell and nuclear encode =

genes in fertile asymmetric somatic   hybrids between Lycopersicon =

esculentum and L. peruvianum.
- evidence for rearrangements in chloroplast genome of this hybrids

Research skills:

Southern, Northern, Western analysis; DNA, RNA, protein isolation; =

molecular cloning, restriction analysis; preparation of constructs for =

protein expression and for antisense and sense plant transformation; =

analysis of transgenic plants; PCR; in vitro mutagenesis; enzyme assays; =

isolation of organells from plants; work with radioisotops and in vitro =

cultures; somatic hybridization; isolation of chloroplast and =

mitochondrial DNA

Scientific interests:

Chlorophyll biosynthesis and its regulation; relation of protein =

structure and function; effect of herbicides on plants; genetic =

ingeneering; genetic transformation of plants; recombination of =

cytoplasmic genomes; nucleo-cytoplasmic interractions; RNA editing in =

plant organells; cancerogenesis.

Languages:

English, Russian, Ukrainian - read / speak.

 List of publications

Ratushnyak YI, Piven NM, Latypov SA, Samoylov AM, Zavgorodnyaya AV, Gleba =

YY (1991) Changes of the genetic constitution of the tomato plants by =

gamma-fusion of Lycopersicon esculentum Mill. and Lycopersicon peruvianum =

var. dentatum Dun. protoplasts. Biopolymers and Cell  7:82-91.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko  IV, =

 Rachkovskaya  RI,  Gleba  YY  (1993)  Cell engineering synthesis of =

tomato fertile asymmetric somatic hybrids by gamma-fusion of protoplasts. =

 Genetika,  29:164-176.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA, Borozenko IV, =

 Rachkovskaya RI,  Gleba YY  (1993)  Fertile asymmetric somatic hybrids =

between Lycopersicon esculentum  Mill. and Lycopersicon peruvianum var.  =

dentatum Dun. Mol. Gen. Genet, 236: 427-432.

Rudas  VA,  Zavgorodnyaya AV,  Cherep NN,  Gleba YY (1993) Genetic =

transformation by leaf-disks method of wild species Solanum and =

Lycopersicon. Biopolymers and  Cell  9:73-77.

Ratushnyak YI, Kochevenko SA, Cherep NN, Zavgorodnyaya AV, Latypov SA, =

Gleba YY (1995) Alloplasmic incompatibility of cybrid plants possest =

nuclear Lycopersicon esculentum and plasmagenes of Lycopersicon =

peruvianum var. dentatum Dun. Genetica 31(5): 660-667.

Zavgorodnyaya AV  (1994) Nucleo-cytoplasmic inheritance in asymmetric =

somatic hybrids in Lycopersicon genus. Ph.D. thesis, Kiev, UKRAINE.

Cherep NN, Zavgorodnyaya AV, Ratushnyak YI, Gleba YY (1995) =

Reconstruction of chloroplast DNA in asymmetric somatic hybrids =

Lycopersicon esculentum Mill. + Lycopersicon peruvianum var. dentatum =

Dun.  Genetica  (in press).

Zavgorodnyaya AV, Grimm B (1996) Yeast 5-aminolevulinate synthase =

provides additional chlorophyll precursor in transgenic tobacco =

(submitted EMBO J.).

Zavgorodnyaya AV, N.N., Grimm B (1996) Influence of gabaculline on =

expression of proteins involved in chlorophyll biosynthesis and =

photosynthesis (manuscript in preparation).

ABSTRACTS:

Piven NM, Ratushnyak YI, Rudas VA, Latypov SA, Zavgorodnyaya AV,  Gleba  =

YY  (1991)  Selection  of  somatic  hybrids in  Lycopersicon and Solanum =

using chlorophyll-deficient mutants and gamma-irradiation.  Abstr  VIII  =

Internl  Protoplasts Symposium, Uppsala, p 28.

Cherep  NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba YY    (1993)  =

Chloroplast  DNA  rearrangement in Lycopersicon asymetric somatic =

hybrids.  Abstract  XVIIth  Intern.  Congress of Genetics, Birmingham, p =

191.

Ratushnyak YI,  Cherep NN,  Zavgorodnyaya AV,  Latypov SA,  Kochevenco =

AS, Gleba YY (1994) Alloplazmic incompatibility in cybrid plant of tomato =

obtained by cell engineerihg methods.  Abstract VIIIth Intern. Congress =

of Plant Tissue and Cell Culture, Firenze, p 101.

Cherep NN,  Zavgorodnyaya  AV,  Ratushnyak  YI,  Gleba  YY  (1994)  =

Organell and nuclear genomes analysis of tomato cybrid plants.  Abstracts =

4th Intern.  Congress  of  Plant  Molecular Biology, Amsterdam, p 153.


LECTURES:

ETH Z=FCrich, 12.8.1996: =84Yeast 5-aminolevulinate synthase provides =

additional chlorophyll precursor in transgenic tobacco=93



References:

Grimm Bernhard, Dr. hab.
Department of Molecular Cell Biology
Institute of Plant Genetics and Crop Plant Research
06466 Gatersleben
Germany
Tel.:  49-39482-5364
Fax:  49-39482-5139
E-mail: grimm at ipk-gatersleben.de

Gleba, Y.Y., Professor, Ph.D.
Director of Institute of Cell Biology and Genetic Engineering,
American Cyanamid Company,
Princeton, NJ 08540, =

U.S.A.
Tel: 1-609-799-0400.
Fax: 1-609-799-1842.
E-mail: glebay at pt.cyanamid.com

Alisher Touraev, Ph.D.
Institute of Mikrobiology and Genetics,
Biocentre of Bohrgasse, Vienna University =

Dr.Bohrgasse 9 =

1030 Vienna =

Austria.
Tel: 1-79515 7820.
Fax: 1-798 62 24.



More information about the Plantbio mailing list