Chloroplast RNA Editing--Postdoctoral Position

Maureen Hanson mrh5 at cornell.edu
Tue Feb 11 10:15:01 EST 1997


Postdoctoral position available to study RNA editing in  vascular plant
chloroplasts.  In dicots and monocots, transcripts of certain genes are
altered by a unique form of RNA processing which converts a C to a U.  A
typical plant species exhibits about 40 locations in chloroplast
transcripts where a C is changed to a U.  The C-to-U alteration usually
alters the encoded amino acid from the one predicted from genomic
sequence.  Little is known about the cis-acting elements which result in
selection of a particular C for editing while other Cs never undergo
editing.  Nothing is known about the trans-acting molecules which may be
involved in site selection and catalysis of the conversion.

Chloroplast genomes can be transformed with deliberately altered
chloroplast genes to study the mechanism of site selection.  Such
experiments have revealed that short regions of sequence surrounding
certain editing sites are sufficient to specify editing.

For a review of RNA editing in chloroplasts and mitochondria, consult
Hanson et al. (1996) Plant organelle gene expression: altered by RNA
editing. Trends in Plant Science 1:57-64.  An example of the chloroplast
transgene approach to studying editing can be found in Sutton et al.
(1995) A plant mitochondrial sequence transcribed in transgenic tobacco
chloroplasts is not edited. Mol. Cell Biol. 15:1377-1381.

The postdoctoral position provides an opportunity to learn tissue culture
and chloroplast transformation methods.  Background in recombinant DNA
technology  is essential, but previous experience with plants is not
required.   The  position can begin between March and July, 1997,
negotiable.

The research laboratory is located in a modern building which contains
several useful research facilities:
Analytical and Synthetic Chemistry Facility: oligonucleotide and peptide
synthesis, automated DNA sequencing, mass spectrometry, peptide sequencing
Flow Cytometry and Microscopy: fluorescence-activated cell sorting and
confocal microscopy
Plant Tissue Culture and Transformation: particle gun, electroporator,
plant tissue culture growth facilities
Computer Facility: networked access to Wisconsin package and DNAStar
software packages.

Many opportunities for further training in plant biology exist at Cornell
University.  Over 100 active plant scientists are on the faculty of eight
different departments.   Twenty faculty form a graduate training program
in the Molecular Mechanisms of Plant Processes, funded by an interagency
(DOE/NSF/USDA) training grant.

Send by mail, fax or email, a c.v. and names of three references to:
Maureen Hanson, Section of Genetics and Development, Biotech. Bldg.,
Cornell University, Ithaca, NY 14853 USA.  email: mrh5 at cornell.edu.  FAX
607-255-6249.



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