6xhis purification

TAMMY ADAIR TAMMYMART at MAIL.HOT1.NET
Tue May 20 15:34:35 EST 1997


I have recently tried to purify 6xhis tagged recombinant proteins from 
Nicotiana benthamiana.Western blots of my control plants show the 
large rubisco subunits also bind Ni as if they contained polyhistidine 
sequences. Does anyone have any info on this? Are there some proven 
affinity tags which work in purifying foreign proteins in plants?
Thanks



More information about the Plantbio mailing list