New Lab Manual Plant Cytology

Hans Sluiman h.sluiman at
Fri Sep 5 05:46:32 EST 1997


Laboratory Manual of Plant Cytological Techniques


Information from chromosomes is of great value to plant taxonomists as
well as to those researchers aiming to conserve genetic resources and
biodiversity. Following the Rio Convention, taxonomy is enjoying a
resurgence of interest and support, and yet there remains an urgent need
for the training of cytologist to provide vital base line data for
taxonomic and systematic studies.There are many countries blessed with
rich and diverse floras, where for the majority of plant species even
basic cytological information such as chromosome numbers is not known.
Although there are many taxonomists who are well able to secure the
cytogenetic data they need themselves, many either do not have the time,
or, perhaps more commonly, the inclination or know-how to try. 

One of the main aims of this Manual is to provide access to some
well-tried protocols, for obtaining good chromosome preparations. The
Manual is targeted at those with no, or little previous experience of
working with plant chromosomes, and provides a clear and concise
introduction to the field. For those who have struggled to get good
chromosome preparations or had difficulty even seeing chromosomes in the
slides they have produced, this guide contains step by step easy to
follow schedules, with many hints and suggestions that will help towards
getting useful cytological data, so that "having another go" becomes
less daunting.

The Manual includes

- a brief introduction to the chromosome squash technique 
- a selection of well-tried step by step pretreatment and staining
schedules for producing a good chromosome slide 
- recipes for the preparation of stains and other reagents 
- suggestions on how to tackle difficult plant groups
- illustrations of stages of mitotic and meiotic cell division 
- information on variation in chromosome morphology and examples of
taxonomic use of cytological data
- hints on recording observations and record keeping
- techniques for preparing slides for permanent storage
- references to original published sources and other relevant

ISBN 1 872291 42 2
Softback, wiro-bound, 210 x 297 mm, vi + 96 pp. 
Price GBP 10.00 + postage (UK: 1.50, overseas: 4.50)

To obtain a copy, send a cheque for GBP 10.00 plus the appropriate
postage to:

Ruth Hollands, Scientific & Technical Services, Royal Botanic Garden
Edinburgh, Inverleith Row, Edinburgh EH3 5LR, UK.

IMPORTANT: Cheques should be made payable to "Royal Botanic Garden
Edinburgh"; they should be in pounds sterling and drawn on a UK bank.
Eurocheques are also acceptable.

For credit card payments (Access/Mastercard/Visa), an order form must be
completed, available upon request from Ruth Hollands
(r.hollands at

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