mmalloy at chickasaw.com
mmalloy at chickasaw.com
Sat Oct 31 13:44:33 EST 1998
Tom, Thank you for your time and information. Where to get the culture has
been a problem for us. I really appreciate your input. That helps! The
articles you listed sound interesting. I am sure they will help us as well.
In article <363A2C70.500E1723 at dtpax2.ncifcrf.gov>,
McCloud at dtpax2.ncifcrf.gov wrote:
> Agerobacterium is a normal soil organism and is not a human pathogen. It can
> purchased from Carolina Biological Supply, Box 6010, Burlington, NC 27216
> carolina at carolina.com catalog number D8-15-4825, tube $7.40 Can be
> maintained on simple nutrient agars, storred and remain viable for weeks in
> refrigerator. This bug will also form galls on sterile discs cut out of
> potatoes, carrots and other vegetables. A decreases in the number of galls
> disc has been used as a predictor of anticancer activity. Take a look at :
> 1) A blind comparison of simple benchtop bioassays and human tumor cell
> cytotoxicities as antitumor prescreens by J.E. Anderson et al, Phytochemical
> Analysis 1991, 2, p.107
> 2)Crown gall tumors on potato discs and brine shrimp lethality: two simple
> bioassays for higher plant screening and fractionation by J.L. McLaughlin in
> Methods in Plant Biochemistry vol.6, 1991
> 3)Crown gall tumor bioassay by A.G. Galsky Plant Physiol. 65, 184, 1980
> 4) Use of potato disc and brind shrimp bioassays to detect activity and
> piceatannol as the antileukemic principle from seeds of Euphorbia lagascae by
> N.R. Ferrigni et al Jol. of Natural Products 47, 347, 1984
> The cost of doing this is low, does not require sophisticated apparatus, and
> should be within the technical reach of a pretty intelligent 6th
> grader. Tom McCloud
> mmalloy at chickasaw.com wrote:
> > Someone please help.
> > My daughter is in the 6th grade. She has expressed interest to me in making
> > science project by demonstration of a Scientific Method for her Science
> > I am trying to help her get started.
> > The subject she picked (plant pathology) is called: "How Effective is Beta
> > Carotene in Fighting Cancer in Plants?".
> > Purpose: To determine whether beta carotene has any substantial effect in
> > reducing or eliminating the presence of Agrobacterium tumefaciens in plants.
> > We have been searching the internet for additional information about this
> > experiment.
> > The best I can tell is that we are experimenting with Crown Gall disease.
> > that correct?
> > The written procedure we have calls for the use of Sun Flowers germinated
> > from seeds that were treated with beta carotene solution but this seems like
> > it might take too long to go all the way through the germination phase as
> > well as the growth, and infection ... phases. The project will be due in 2
> > months.
> > I was wondering if anyone reading this could help with advice and/or
> > suggestions on the project like:
> > 1. An alternative plant (fast growing and easy to infect) that we could get
> > from a local florist or green house. One thing I read said to use tomato
> > plants. Would this work ok? What other plants might be good for this time
> > the year grown inside with a plant light like ordinary house plants in two
> > months time?
> > 2. A source for the Agrobacterium tumefaciens culture: I have only been
> > able to find one source - on the internet - which was posted on a bionet
> > forum leading to: www.atcc.org, which turns out to be a supply house for
> > cultures, but they have 20 modified strains of Agrobacterium tumefaciens to
> > choose from, some of them would cost over $100.00 !? Which one should be
> > used for a 5th grade science project? Am I looking in the wrong direction?
> > I am not a genetic engineer nor is my daughter. I haven't tried to purchase
> > culture yet.
> > 3. A source for the beta carotene in semi-pure form (reagent) that could be
> > measured, mixed and diluted to desired strength.
> > 4. Any tips on how to insure that the procedure might work. Alternative
> > methods, safety, etc.
> > 5. I was wondering if Agrobacterium tumefaciens cultures are considered
> > bio-hazards and could we even buy it without lab qualifications?
> > Please E-mail comments
> > ANY information on this subject would be of help.
> > mmalloy at chickasaw.com
> > Thank you for your help.
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