Determining if a root is dead
koning at ECSUC.CTSTATEU.EDU
Thu Feb 11 18:34:18 EST 1999
At 3:09 PM 2/11/99, Bruce K. Kirchoff wrote:
>Does anyone know of a vital stain that would help me determine if a root
>is dead? I have been asked to give my professional opinion (I am am
>plant morphologist/anatomist) on whether a root mass growing (and
>clogging) a sewer is dead. It was treated with a standard herbicide
>about 6 months ago. Based on it's external appearance the roots appear
>dead, but, as there is a considerable amount of money involved in this
>project, I have been asked to give my professional opinion. Can anyone
>help me? Does anyone know a definitive test for living tissue?
> Bruce Kirchoff
> University of North Carolina at Greensboro, USA
> kirchoff at uncg.edu
WOW it has been FOREVER ! I hope things are going well for you.
My suggestion is to try a tetrazolium test on some fresher
looking areas...it basically tests for respiratory metabolism.
Cellular metabolism also prevents uptake of Evans Blue dye,
so living cells should exclude it.
\ Ross Koning \
\ Biology Department \
\ Eastern CT State University \
\ Willimantic, CT 06226 USA \
\ Koning at ecsu.ctstateu.edu \
\ http://koning.ecsu.ctstateu.edu \
\ Phone: 860-465-5327 \
) Fax: 860-465-5213 )
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