Acid Rain

Kee-Shik Moh plantlet at
Tue Oct 19 11:26:03 EST 1999


   Study was carried out to determine the effects of simulated acid rain on
root growth and development of Phaseolus vulgaris seedlings. Simulated acid
rain was prepared by diluting 0.1N sulfuric acid with tap water and adjusted
to pH 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 and 5.7. Tap water (pH 6.7) was used
as control. At pH values below 3.0, plants exhibited a failure to obtain
normal rooting, the pH 4.0-5.0 had necrotic and wrinkled roots, and lateral
root was inhibited at pH 4.0, 4.5 and 5.0. The seedling treated with tap
water showed normal root development and normal structure of metaxylem and
secondary xylem.
   Total proteins subjected to SDS-PAGE to analize changes of protein
pattern occurred during the root development of Phaseolus vulgaris
seedlings. From one-dimensional SDS-PAGE, several protein bands could be
identified which were specific for (65KD, 48.0KD, 42.4KD,41KD, 38.4KD and
35.8KD) pH 2.0 solution. The bands of 185.2 KD, 64.5KD, 44KD, 41.9KD, and
36.1KD proteins were common at pH 4.0, pH 5.7 and pH 6.7. Thus the protein
bands of 33.1 KD appeated in root treated with acid rain of pH 5.7 and pH
6.7. The protein patterns were different according to the pH during the root
growth of Phaseolus vulgaris seedlings.

Phaseolus vulgaris ; kidney bean

A thesis of graduate

Subject : The effects of simulated acid rain....

Kee-Shik Moh,...
Department of Biology
Graduate School
Chonbuk National Univ.

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> How does the sulphuric acid in acid rain effect plants? What signs
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