Internal standards for RT-PCR

David Humair david.humair at rac.admin.ch
Fri Aug 11 09:25:06 EST 2000


Hi netters,
I want to quantify the expression (in fact decrease) of an mRNA in
senescing plants. Regarding at some commercial kits (generally for
animal cells) people use: beta-actin, 18S RNA, GAPDH as standards. I
have also heard ubiquitin. Has anybody heard things about that. Does
anybody work with the QuantumRNA kit from Amplion (competimer technique)
with plant material?
Do you know if the expression of these standards decreases with the
early senescence of the plant cells?

Best regards

David Humair


--
Dr. David Humair
Station fédérale de recherches en production végétale de Changins
Laboratoire de génie génétique
Route de Duiller
CH-1260 Nyon

Tél : +41-22-3634419
Email : David.Humair at rac.admin.ch


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