Internal standards for RT-PCR

bovet bovet at sis.unibe.ch
Fri Aug 18 02:34:15 EST 2000



David Humair wrote:

> Hi netters,
> I want to quantify the expression (in fact decrease) of an mRNA in
> senescing plants. Regarding at some commercial kits (generally for
> animal cells) people use: beta-actin, 18S RNA, GAPDH as standards. I
> have also heard ubiquitin. Has anybody heard things about that. Does
> anybody work with the QuantumRNA kit from Amplion (competimer technique)
> with plant material?
> Do you know if the expression of these standards decreases with the
> early senescence of the plant cells?
>
> Best regards
>
> David Humair
>
> --
> Dr. David Humair
> Station fédérale de recherches en production végétale de Changins
> Laboratoire de génie génétique
> Route de Duiller
> CH-1260 Nyon
>
> Tél : +41-22-3634419
> Email : David.Humair at rac.admin.ch
>
> The hardness of the butter is proportional to the softness of the bread.

Dear Colleague,

Lets try the protein 16S. It is an excellent constitutive gene for your
application!

Dr Lucien Bovet

PS: Comme on se retrouve! Epatant non! Au plaisir de te revoir (tes mails me
manquent à Neuch!)







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