How to quantify antisens mRNA

Frank Ludewig frank.ludewig at
Fri Nov 3 07:16:36 EST 2000

You can distinguish just by choosing "differential" primers, for that you
shouldn`t have taken the complete cDNA for antisense expression, or (if you
want to "quantify" your transgene) choose primers sitting on the MCS of your


Dr. Frank Ludewig
Department of Botany II
University of Cologne
Gyrhofstrasse 15
50931 Cologne

email: frank.ludewig at
Fax:   49-(0)221-4705039
Phone: 49-(0)221-4703388

David Humair <david.humair at> schrieb in im Newsbeitrag:
3A029879.F855B75 at
Hi all,
I have a theoretical problem with RTPCR on antisens mRNA. How can I
distinguish between the antisens mRNA and the endogenous sens mRNA?

Thanks for your help

Dr. David Humair
Station fédérale de recherches en production végétale de Changins
Laboratoire de génie génétique
Route de Duiller
CH-1260 Nyon

Tél : +41-22-3634419
Email : David.Humair at

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