Subtracted Libraries

Barry Flinn bflinn at
Wed Sep 27 13:29:34 EST 2000

I'm looking for information from anyone regarding non-PCR based methods for
producing subtracted libraries.  Specifically, I know that Dynal suggests
the use of
oligo-dT beads to make subtracted libraries, but I can't find a protocol
Does anyone have any information or a protocol regarding the use of Dynal
for the above purpose, or any other non-PCR based protocols?

Thanks for any help you can provide.

Barry Flinn


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