puec.bbs at flight.bio.ncku.edu.tw
Tue Jan 16 12:45:02 EST 2001
Recently I got a gene from datas released by Arabidopsis
Genome Project. I used Arabidopsis cDNA library as template
and the 3' and 5' end sequences plus restriction sites as
primers. However, I can't detect any bands after 30 cycles
PCR and electrphoresis on the gel.
Now I want to find it's promoter and creat a transgenic
plant harboring promoter::GUS to observe its expression
But I don't know how to distinguish between introns and
promoters. I know the TATAAT and TTGACG but I don't think
that it was reliable enough.
Are there more clues for me to find the promoters?
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