help for confocol

Baichen Zhang Zhang at
Thu Sep 12 01:21:40 EST 2002

Dear All,

I tried to use the confocol microscopy to observe the carboxylfluorescein labelled phloem, though the green to yellow fluorescence is really strong under normal fluorescence microscopy, when I tried to use confocol microscope exciting with 488 nm, there is very weak signal. Could you help to tell how to observe it in a right way?

ANY suggestion will be highly appreciated,


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