arabidopsis nuclei isolation

[vekcna]

I’m looking for a good method for arabidopsis nuclei isolation 
So far I tried using buffer containing hexylene glycol and 0.2% triton
For percoll gradient and couldn’t see shi%$#... in the 80 % layer.
I suspect that somehow I damaged the nuclei 
If anyone has any idea of what is wrong or an alternative method I
would be grateful.


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vekcna
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