Hi,
I'm afraid I can't see the gel pic, but I also use Trizol for RNA preps
from plants (in my case, a legume). Occasionally I see something that
stains w/ EthBr and remains in the well of the gel (otherwise, the RNA
looks fine on a denaturing agarose gel). I've used these preps for
northerns with no problems. Recently, I've been making cDNA and have
gotten somewhat reduced efficiency (compared to other people in lab).
I don't know if this high molecular weight "stuff" is the cause of the
reduced efficiency.
I've read that after a trizol RNA prep, you can further purify by
running the sample through a Qiagen RNA kit, but I've not tried this myself.
Good luck!
Colby
gugu1357 at yahoo.com.br wrote:
>> I am extracting RNA from rice leaves using Trizol . The RNA looks like good
>> but I got a bright band that run above the wells. I do not know what is
>> that. I hope you can see the picture below. I will construct a cDNA library with this RNA but I am afraid this bright band can mess up everything.
>> Could you please give me your opinion in this respect?
>> Thank you for your attention. Angela
>>http://biowww.net/mynews/tree.php?group_name=bionet_plants&begin=0
