I have been trying to get a decent southern using the
DIG labeling system from Roche.THough I get a good labeling
efficiency when I check my probe (labeled using PCR)when I
probe my blot I end up with a very dirty background
My bands are visible though.
COuld it be due to Et bromide ??
Or is it because I am not using a very efficient technique when actually
incubating the membrane with the substrate(CDP star)in the final step.
I try to make sure there are no wrinkles or air bubbles...but...:((