[Plant-biology] Re: Extracting chlorophyll, carotenoids, anthocyanin, etc

Kyle Bender bender.kw at gmail.com
Tue Jan 31 15:28:00 EST 2006


I just finished a lab in one of my university classes involving pigment 
extraction.  You can use acetone or 80% ethanol to extract the pigments. 
  Once chlorophyll is extracted everything else is too.  Use 
chromatography paper to separate out the pigments...the only separation 
you might not get is the separation of Chl a and b.  Once pigments are 
separated on the chromo paper cut the strips and redissolve the 
pigments.  From there you can use your spec to get absorbance readings.

Hope that helps

Kyle


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