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[Plant-biology] NOS promoter

Vasudha Chauhan via plantbio%40net.bio.net (by vchauhan from ucdavis.edu)
Thu Aug 29 15:23:19 EST 2013


Hello everyone,

I have a construct that contains the NOS promoter which controls the Hyg
resistance gene followed by a NOS terminator. This construct also contains
TALENs. However, I have heard that NOS promoter has given trouble in the
past in Arabidopsis when transforming with agrobacterium. Does anybody have
any experience with transformation with a construct that contains pNOS? If
yes, then has anybody done this in other crops, such as tomato?

Thank you
Vasudha

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