shifting the heavy chain of IgG

Jianyin Long long at cabm.rutgers.edu
Thu Oct 4 22:28:42 EST 2001


Hi, there,
I am doing immunoprecipitation followed by an immunoblot assay. My target 
protein(53kD) comigrates with the heavy chain of IgG(~50kD). Except IP/IB 
in the other way, does anyone has the experience of shifting the heavy 
chain to a higher position? I read from literature that N-ethylmaleimide 
has such an effect, but do not know the detailed protocol.
Thank you all for your possible help.
Jianyin




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