shifting the heavy chain of IgG
long at cabm.rutgers.edu
Thu Oct 4 22:28:42 EST 2001
I am doing immunoprecipitation followed by an immunoblot assay. My target
protein(53kD) comigrates with the heavy chain of IgG(~50kD). Except IP/IB
in the other way, does anyone has the experience of shifting the heavy
chain to a higher position? I read from literature that N-ethylmaleimide
has such an effect, but do not know the detailed protocol.
Thank you all for your possible help.
More information about the Plsignal