anomalous protein migration on PAGE ?

37_410 at uwovax.uwo.ca 37_410 at uwovax.uwo.ca
Wed Sep 5 19:39:49 EST 1990



Does anyone have any experience with proteins that migrate significantly slower
by SDS-PAGE than their DNA sequence predicts?  I have a 22.3 Kd ORF that yields
a 35 Kd polypeptide on gels.  I would like to know if someone has similar
experience or some possible explanation(s).

Jim Gordon
University of Western Ontario,
Ontario, Canada
email: (care of) T.LINN at uwovax.uwo.ca



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