missense mutants

Doug Prasher prasher at hyatt
Thu Aug 1 10:29:25 EST 1991


Fellow Netters,

What is the current opinion about which is the best E.coli genotype
for the production of missense mutant forms of eucaryotic proteins?

We are working with several derivatives of a eucaryotic protein
(the photoprotein aequorin), each of which is produced in E.coli
at levels significantly below that of a wild type form.  Since I
assume the problem is due to protein degradation, we intend to
try using strains having other genotypes (eg. lon, htpR).  If you have 
experience with this type of problem, please send your comments.

I will summarize the responses and repost them if requested.

So long, farewell.

********************************************************************
Douglas Prasher				*  prasher at hyatt.whoi.edu
Woods Hole Oceanographic Institution	*  508-457-2000 ext 2311
Biology Department			*  FAX: 508-457-2195
Woods Hole, MA 02543			*
********************************************************************



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