Chromatography on Heparin-sepharose

[Dan Diaz]

I am having a devil of a time getting my enzyme (an exonuclease)
to behave consistently on Hep-Seph CL-6B (Pharmacia).  On some
home-made H-S the enzyme eluted at about 250 mM NaCl.  Using the
Pharmacia material, the enzyme elutes much later (closer to 350
mM.  I would appreciate any email from other protein types who
have used heparin chromatography.