peptide analysis

[srckuxw at lincoln.cri.nz]

Dear colleagues

I am interested in the glycosylation of a protein. My idea is to separate a
tryptic (or other) digest of my protein on a polyacrylamide gel, blot them on a
PVDF Membrane and screen the membrane afterwards for glycosylated peptides. Has
anybody experiance with that kind of work. I am manly interested in hints how
to bind small peptides (smaller than 10K) on membranes.
Thanks in advance
                         Urs Waefler
                         Crop & Food Research
                         Lincoln
                         New Zealand