Request help on subunit crosslinking

Dan Diaz bl275 at cleveland.Freenet.Edu
Thu Oct 1 20:24:43 EST 1992


I am trying to ascertain the subunit structure of E. coli exonuclease VII,
which is composed of 52 and 9 kDa subunits in an approximate 1:4 ratio.  I
would like to try glutaraldehyde crosslinking and would appreciate any
feedback on methods, protein concentration, NaBH4 quenching,
troubleshooting, etc.
-- 
Dizzy Dan         ddiaz at cwru.bitnet      bl275 at cleveland.freenet.edu
Dept. Biochemistry & Wine Tasting  CWRU School of Medicine & Sushi
"I usually like to live in the past, but my time machine is broken"



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