Increasing pH and temperature stability of enzymes

TAO BRIAN T 90taobri at
Thu Jul 8 12:23:29 EST 1993

    Does anyone have experience in inducing point mutations in an enzyme
in order to increase its thermal or pH stability?  I have two enzymes,
each from a different bacterial species.  One has a wide pH range of
activity (3.5 to 8) and is stable to about 60 C.  The other has a
narrower pH range, is degraded above 40 C, but it has 3 to 5 times the
acitivity of the first.  Are there any guidelines or hints for mutating
either enzyme so the benefits of both are present in the mutant? 
References would be greatly appreciated.
Brian Tao:: taob at (Internex Online, 416-363-3783, 10 lines, v.32bis)
::::::::::: *** Note that my <taob at> address has changed ***

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