Cloning pg amouts of DNA?

Seth Daniel Crosby dd034 at cleveland.Freenet.Edu
Fri Jul 9 16:26:15 EST 1993


I have developed a technique for immunoprecipitation of chromatin
using an anti-transcription factor polysera.  It works well as
indicated by PCR detection of a known target gene.  I would like
to clone other fragments in order to determine other candidate
target genes.  

The problem is the technique yields picogram quantities of DNA.
How do I clone vanishingly small amounts of chromatin!?



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