those pesky glycoprotein stains...
quinones at biology.ucsc.edu
Tue Jul 13 18:06:05 EST 1993
Here's another question regarding this thymol-sulfuric acid protocol I am
bent on using. The following is a direct quote from the source of the
said protocol (Methods in Enzymology. 1990. vol. 182, page 537):
3. Immerse the gel in the above solution containing 0.2% (v/v)
thymol (Sigma) for 90 minutes with gentle rocking......
By the way, the "above solution" is a 2-propanol/acetic acid fix.
I dutifully placed my order with Sigma, and what I got (all they sell)
is thymol in crystal form. My question is: is the "v/v" a typo? In any
case, could anybody out there tell me how to make it through step 3 ???!
Maybe, someday, I will actually dare to run these gels....
[and, in case the machine does its usual trick, my address is
quinones at biology.ucsc.edu ]
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