information on bandshifts

Dan Kolk UCSD
Tue Jul 20 14:15:09 EST 1993


	Many things can cause smearing in band shifts.  I suggest; 1) going with
29:1 acrylamide:bis, there really is no reason to go with 80:1. 2) Try as
simple a reaction buffer as possible i.e. 25mM Hepes pH 7.9, 100mM KCl, 5
mM MgCl, 12% glycerol, 1mM EDTA, 1mM DTT. 3) Dialysing your nuclear
extracts and adding protease inhibitors. 4) Run your labelled probe on a
sequencing gel to make sure it is not degraded. 5) Make up new running
buffer and filter it.  



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