isoelectric focusing

[liang xia]

Dear netters:

	I have been using isoelectric focusing gel to analyze a herpes immediate-early
protein-ICP4. The major problem I encountered was the poor solubility of the
protein in urea solubilization buffer. Is there anyway to enhance the solubility
of proteins in this buffer or are there any alternative buffers for this
purpose?
Thanx.
Liang
lxia at cor-mail.biochem.hmc.psu.edu
Hershey Medical Center