problem with elution from hydrophobic column

bioc07 at otago.ac.nz bioc07 at otago.ac.nz
Sun Mar 14 05:13:56 EST 1993


In article <1993Mar9.140331.203720 at uctvax.uct.ac.za>,
blatch at uctvax.uct.ac.za writes:

> PROBLEM WITH ELUTION CONDITIONS FOR A HYDROPHOBIC COLUMN: 
> 
.
.
.
> hydrophobic moiety? Any suggestions will be greatly appreciated.
> Please send suggestions to me or Neil at micro.uct.ac.za 
> 
> 
> 

You might try using a non-ionic detergent such as Tween 20 to elute
your material. I have had success with this on Pharmacia
Phenyl-superose columns. The inclusion of 0.02% Tween 20 in the
elution buffer has both given me better yields of some proteins and
removed others that otherwise needed acetonitrile to get them off the
column. The elution position will be somewhat advanced but this need
not be a problem.

--
        Craig Marshall          bioc07 at otago.ac.nz                          
        Biochemistry Department 
        University of Otago     Phone 64 3 479 7849   	
        P.O. Box 56             Fax   64 3 479 7866   	
        Dunedin, New Zealand                                   	



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