mutator strain or plasmid

M.J. Horsfall mjhf at troi.cc.rochester.edu
Sun Nov 7 11:52:34 EST 1993


In <David_Nunn-061193122507 at tsikar1.life.uiuc.edu> David_Nunn at qms1.life.uiuc.edu (David Nunn) writes:

> I've got either a bum strain of EC LE30 (mutD) or it just isn't doing the
>trick (mutagenesis-wise). I've been good and kept it on minimal media-only
>one transfer since receiving it-but it still is not working. Maybe just
>need a new strain?

In minimal media, mutD can increase the background mutation frequency some
500-fold, but in rich media (i.e., LB) this increase is something like
50,000-fold. Are you doing your mutagenesis in rich media?

see Schaaper, 1988 (PNAS 85:8126); Schaaper and Radman (1989) EMBO J. 8:3511

Does this help? Hope so.

Mike
--
Michael Horsfall Ph.D.             ((           mjhf at troi.cc.rochester.edu
Department of Biophysics            ))          Phone:(716)-275-8703 (Lab)
University of Rochester     5'-GCAAGTCGGAG-3'     Fax:(716)-275-6007(Dept)



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