Crystallization of Membrane Proteins

schaf at cgl.ucsf.edu schaf at cgl.ucsf.edu
Mon Nov 8 22:25:30 EST 1993


I think it's a "wait and see" kind of thing.

We are still developing the technique, we haven't been
able to set up any crystallization trials yet.

Currently we are trying to develop peptides that form
stable peptide/protein aggregates in order to set up crystal
trials.  We have a peptide that is considerably better
than the one described in the paper but it still isn't good enough,
meaning that at a peptide/BR ratio of 400:1 aggregation stops
and I get an essentially monomeric species with a molecular
weight of ~85kD (one BR ~20 peptides) but that is too much peptide
to concentrate down for crystallization trials.

.Chris.
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Christian E.A.F. Schafmeister               Biophysics graduate student
University of California, San Francisco      UUCP: ucbvax!ucsfcgl!schaf
"Biophysics . . . THE future."             INTERNET: schaf at cgl.ucsf.edu


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