Forwarded post

ricke at rcf.mayo.edu ricke at rcf.mayo.edu
Mon Nov 8 17:48:25 EST 1993


Posting on behalf of M. G. Smyth & P. Gunning    - D. Ricke

From:	SMTP%"gunnip at essex.ac.uk"  5-NOV-1993 08:02:52.46
Subj:	Assistance required
From: P Gunning <gunnip at essex.ac.uk>

Subject: Electroelution & Silver-Staining

Q1. Can anyone tell me how to electroelute a protein ( MW 146 kDa,) from a urea 
gel ( 8M?) I've tried the usual electroelution methods with no success.

Q2. The quickest silver staining method I know is the one by Heukeshoven and 
Dernick, 1985, electrophoresis 6:103.) I did try a rapid stain from Biorad but 
this method was not as good as the one by H&D. Can anyone recommend 
alternatives?

Please reply to : smyth at afrc.ac.uk, because I don't have access to USENET/BIONET

Thanks,

Martin G. Smyth

________________________________________________________________________________
Martin G. Smyth
AFRC Institute for Animal Health
Compton Laboratory
Berkshire RG16 0NN
UK

Ph: +44 635 578411 ext 541 ( Laboratory, Mon-Fri 0900 to 1700 GMT )
    +44 635 578888         ( Laboratory, outside above hours )
    +44 635 578137         ( Home )

e-mail: smyth at afrc.ac.uk

********************************************************************************

Many thanks,

Paul Gunning
Physics Department
University of Essex
Colchester CO4 3SQ



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