Help: Supershifting bZIP protein in EMSA

Dan Kolk dkolk at popmail.ucsd.edu
Tue Sep 21 12:22:17 EST 1993


	I am attempting to supershift a bZIP protein that we have cloned.  We know
the antibody is good ( it works in western blots and in
immunoprecipitations.)  I have had no luck.  Are there any good references
out there for supershifts?
How much antibody do you added the reaction mixture? How long do you
incubate it? Do you alter the reaction conditions?  Any advice would be
appreciated. 



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