E.Coli Westerns

Dennis Goode GOODE at ZOOL.UMD.EDU
Thu Dec 1 10:37:34 EST 1994

 dan_szymanski at qms1.life.uiuc.edu ("Dan Szymanski")
on the Subject:        E.Coli Westerns asked:

> A couple of us have been trying to detect recombinant E.Coli proteins
> with rabbit polyclonal Ab.  Backround is horrendous.  Does anyone have
> an explanation or suggestions to reduce backround?
> thanks,
> dan 

Also, try absorbing your polyclonal antiserum first with non-
recombinant E. coli and/or E. coli proteins. If that doesn't help, 
you'll probably have to go to monoclonals.


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