E.Coli Westerns

[Dennis Goode]

 dan_szymanski at qms1.life.uiuc.edu ("Dan Szymanski")
on the Subject:        E.Coli Westerns asked:

> A couple of us have been trying to detect recombinant E.Coli proteins
> with rabbit polyclonal Ab.  Backround is horrendous.  Does anyone have
> an explanation or suggestions to reduce backround?
> thanks,
> dan 
> 
Dan:

Also, try absorbing your polyclonal antiserum first with non-
recombinant E. coli and/or E. coli proteins. If that doesn't help, 
you'll probably have to go to monoclonals.

-DG
>