Signal Seq. Trap

schren13 at uctvax.uct.ac.za schren13 at uctvax.uct.ac.za
Tue Jan 4 10:08:54 EST 1994


I am looking for a SIGNAL SEQUENCE TRAP
CAN ANYONE HELP ?

The plasmid-trap I have in mind should have the following features:

1)	ori for replication in E.coli
2)	medium copy number in E.coli
3)	antibiotic resistance marker
4)	upstream E.coli -10/-35 promoter
5)	multiple cloning site downstream from promoter
6)	signal-minus indicator gene downstream from cloning site (eg phoA)

Should anyone know of such a construct please let me know. My e-mail address is
rscholle at physio.uct.ac.za




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