Protein isolation

ragnar.flengsrud at ibf.nlh.no ragnar.flengsrud at ibf.nlh.no
Mon Jan 10 14:59:46 EST 1994


In article <2gqdmv$ed7 at nntp.ucs.ubc.ca> sarven <sarven at ecc.ubc.ca> writes:
>Hi!
>
>Could someone out there be kind enough to send me (or give me the
>reference for) a reliable, quick protocol for isolating total protein
>from tissues for Western blots.  
>
>Thanks
>
>Sarven
>

Hi, 
My suggestion is that you start with Methods in Enzymology, volume 182: Guide
to Protein Purification. 
Special attention should be payed to pp. 425-495 (Purification Procedures:
Electrophoretic Methods.

Hope this helps.
Regards,
Ragnar Flengsrud, Dept of Biotechn. Sci., Agricultural University of Norway.



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