Protein isolation

[ragnar.flengsrud at ibf.nlh.no]

In article <2gqdmv$ed7 at nntp.ucs.ubc.ca> sarven <sarven at ecc.ubc.ca> writes:
>Hi!
>
>Could someone out there be kind enough to send me (or give me the
>reference for) a reliable, quick protocol for isolating total protein
>from tissues for Western blots.  
>
>Thanks
>
>Sarven
>

Hi, 
My suggestion is that you start with Methods in Enzymology, volume 182: Guide
to Protein Purification. 
Special attention should be payed to pp. 425-495 (Purification Procedures:
Electrophoretic Methods.

Hope this helps.
Regards,
Ragnar Flengsrud, Dept of Biotechn. Sci., Agricultural University of Norway.