Recycling Cyanogen bromide activated sepharose affinity columns?
szsclark at hamlet.ucdavis.edu
Thu Jan 27 21:22:43 EST 1994
Dear Netters - I am purifying antibodies from a polyclonal prep by
running them over a CNBr-activated Sepharose affinity column of my
antigen. As CNBr-activated Sepharose is expensive &/or horrible to make,
I'd like to strip the column of the antigen currently bound, and re-use
the sepharose to make another affinity column. Does anyone have a protocol
to do this? Would eluting the column with harsh eluents (eg.
Guanidine/urea/SDS) work? and would I then be able to re-use the sepharose
to couple another antigen? Any advice appreciated. Cheers.
szsclark at ucd.edu
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