Electroanalitical methods

[Emile Schiltz]

Mauricio Cesar Palmieri (mcpalmie at fox.cce.usp.br) wrote:
: Dear Frriends, 
: I am looking for help to find some reference about pKa determination
: of proteins an polypeptides by Electroanalitical methods.
: Any informations will be appreciated.
 


: Thanks in advance.

: ------------------------------------------------------------------------
: Mauricio Cesar Palmieri
: Mestrando em Biotecnologia
:  
: UNIVERSIDADE DE SAO PAULO
: BRAZIL 

: E - mail: mcpalmie at fox.cce.usp.br

: Letters to:
: Universidade Estadual Paulista
: Depto. Bioquimica
: R. Prof Francisco Degni s/n
: Araraquara - SP - Brazil
: CEP 14800-900

hello Mauricio,
the ways one thinks of for pKa-determination for proteins are
- isoelectric focussing
- capillary electrophoresis
- chromatofocussing

hope this helps
Mil
--
Emile Schiltz <eschiltz at sun1.ruf.uni-freiburg.de>
           or <schiltz at bio2.chemie.uni-freiburg.de>
Institute of Organic Chemistry and Biochemistry
Albertstrasse 21, D-79104 Freiburg, Germany
phone: +49 761 203 6090