Phosphorylation of Proteins - Detection?

acer forrest at biosci.uq.oz.au
Fri Jul 8 02:06:58 EST 1994


G'day all,
	Does anyone know of a NON-radioactive procedure for testing whether a 
protein is phosphorylated or not? We are interested in whether our recombinant
is being phosphorylated when expressed in baculovirus. We've thought about using
P32 in the culture medium but this is a messy and dangerous technique, does
anyone know of a non-radioactive technique for determining whether our protein 
is phosphorylated?

Thanks for any replies,
			Alistair Forrest

forrest at florey.biosci.uq.oz.au



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