QUESTIONS: alpha-helix "signals" in proteins

murphy kenneth p kpmurphy at blue.weeg.uiowa.edu
Fri Jul 8 09:38:02 EST 1994


In article <2vj0dsINN2qfk at sat.ipp-garching.mpg.de>,
Richard Engh <engh at nmrvex.biochem.mpg.de> wrote:
>
>Sorry if this point has become peripheral, but regarding thermodynamic
>vs. kinetic control, I think the serpins are relevant:
>
>
>murphy kenneth p (kpmurphy at blue.weeg.uiowa.edu) wrote:
>
>: But isn't there a cleavage involved in obtaining the "latent" state?  This 
>:really difines a new system and it's entirely possible that the global minimum
>: is differen in this new system.  I really don't see the serpin example as 
>: directly addressing the issue of thermodynamic vs kinetic "control".
>
>The serpin plasminogen activator inhibitor-1 becomes latent WITHOUT any
>cleavage.  The latent structure is similar to other cleaved serpin structures,
>except that is it not cleaved.  Prior to the conversion to latency, it is
>in a conformation which is different, possibly flexible or mobile, which is
>an inhibitory form.  The only factors which influence the global minimum
>in vivo will be complexation with ligands (e.g. vitronectin) or other environ-
>mental factors.  The protein in vitro shows kinetic control in that there
>is a significant lifetime for the inhibitory state, before the irreversible
>final folding to a more global minimum latent state.  I don't know what the
>current opinion is regarding the in vivo situation, where further factors
>will influence both kinetics and thermodynamics.
>
>

Thanks for the information.  I wasn't aware that there was no cleavage in this
system.

Kip

-- 
Dr. Kenneth P. Murphy				e-mail: k-murphy at uiowa.edu
Department of Biochemistry			office: (319)335-8910
Univeristy of Iowa				lab:  (319)335-7936
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