Behaviour of Isoenzymes

Martin Tiznado ERNESTO at VM.CC.PURDUE.EDU
Wed Jul 13 14:18:12 EST 1994


I am working in Pectin Methyl Esterase and I had observed that when I use  low
pH (2.5) to elute the protein from an affinity column, I always lost one of the
 proteins. This phenomenon does not happen with another procedures. Any ideas a
bout. Hint: Those proteins can be glycosilated.



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